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CXCR4 Monoclonal antibody
CXCR4 Monoclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Mouse / IgM
Reactivity
human, mouse
Applications
FC, IHC, WB, ELISA and More (2)
Conjugate
Unconjugated
CloneNo.
4B5E4
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, K-562 cells, Daudi cells, Ramos cells, Jurkat cells, U-937 cells, THP-1 cells |
| Positive IHC detected in | human tonsillitis tissue, mouse brain tissue, human prostate cancer tissue, human breast cancer tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | Jurkat cells |
Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
Planning an IHC experiment? We recommend our IHCeasy CXCR4 Ready-To-Use IHC Kit. CXCR4 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60042-1-Ig targets CXCR4 in WB, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | FC, IHC, WB, ELISA |
| Cited Applications | WB, IF, IHC, CoIP |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | CXCR4 fusion protein Ag1528 种属同源性预测 |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | chemokine (C-X-C motif) receptor 4 |
| Synonyms | CD184, CXC R4, CXCR 4, CXCR4, CXCR4B, D2S201E, FB22, Fusin, HM89, HSY3RR, LAP3, LCR1, LESTR, SDF 1 receptor, WHIM |
| Calculated Molecular Weight | 352 aa, 40 kDa |
| Observed Molecular Weight | 60~70 kDa |
| GenBank Accession Number | BC020968 |
| Gene Symbol | CXCR4 |
| Gene ID (NCBI) | 7852 |
| RRID | AB_2091809 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Caprylic acid/ammonium sulfate precipitation |
| UNIPROT ID | P61073 |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
C-X-C chemokine receptor type 4 (CXCR4) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609). CXCR4 has a calculated molecular weight of 40 kDa. In addition to the predicted species of 45-47 kDa for glycosylated CXCR4 monomers, due to ubiquitination, dimeration, and different degrees of glycosylation, additional species can also exist and have been reported in some researches, including 67 kDa (PMID: 23917520), 55, 67, 87 kDa (PMID: 20028517), 80 kDa (PMID: 10506573), 47, 50, 62, and 98 kDa (PMID: 16204649).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CXCR4 antibody 60042-1-Ig | Download protocol |
| IHC protocol for CXCR4 antibody 60042-1-Ig | Download protocol |
| FC protocol for CXCR4 antibody 60042-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun HIF-1α-PDK1 axis-induced active glycolysis plays an essential role in macrophage migratory capacity. | ||
J Exp Med CXCR4 regulates Plasmodium development in mouse and human hepatocytes.
| ||
Nanoscale Mitomycin C-treated human-induced pluripotent stem cells as a safe delivery system of gold nanorods for targeted photothermal therapy of gastric cancer | ||
ACS Appl Mater Interfaces Surface Functionalization of Polymeric Nanoparticles with Umbilical Cord-Derived Mesenchymal Stem Cell Membrane for Tumor-Targeted Therapy. | ||
Mol Oncol Growth arrest-specific protein 2 (GAS2) interacts with CXCR4 to promote T-cell leukemogenesis partially via c-MYC |
