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COX6C Polyclonal antibody

COX6C Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 11429-2-AP

Print datasheet

Synonyms

COX6C

验证数据展示

  • WB analysis of human heart using 11429-2-AP

    WB analysis of human heart using 11429-2-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 11429-2-AP (COX6C antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 11429-2-AP

    IHC staining of human pancreas cancer using 11429-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11429-2-AP (COX6C antibody) at dilution of 1:50 (under 10x lens).

经过测试的应用

Positive WB detected inhuman heart tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11429-2-AP targets COX6C in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen COX6C fusion protein Ag1982 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytochrome c oxidase subunit VIc
Synonyms COX6C
Calculated Molecular Weight 75 aa, 9 kDa
Observed Molecular Weight 9 kDa
GenBank Accession NumberBC000187
Gene Symbol COX6C
Gene ID (NCBI) 1345
RRIDAB_2276720
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP09669
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cytochrome c oxidase (COX) is the terminal oxidase in the respiratory chain in human and other mammalian cells. The enzyme is composed of 13 different subunits. COX6C(Cytochrome c oxidase subunit 6C) is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

实验方案

Product Specific Protocols
WB protocol for COX6C antibody 11429-2-APDownload protocol
IHC protocol for COX6C antibody 11429-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Biol Chem

PMID: 20870728

Authors - Oliva Claudia R CR
View Article