CD86 (C-terminal) Polyclonal antibody

CD86 (C-terminal) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 26903-1-AP

Print datasheet

Synonyms

Activation B7 2 antigen, B7 2, B70, CD28LG2, CD86, CD86 (C-terminal), CD86 molecule, CTLA 4 counter receptor B7.2, FUN 1, LAB72



经过测试的应用

Positive WB detected inDaudi cells, Raji cell, Ramos cells, RAW 264.7 cells, LPS-treated RAW 264.7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26903-1-AP targets CD86 (C-terminal) in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen CD86 (C-terminal) fusion protein Ag25432 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD86 molecule
Synonyms Activation B7 2 antigen, B7 2, B70, CD28LG2, CD86, CD86 (C-terminal), CD86 molecule, CTLA 4 counter receptor B7.2, FUN 1, LAB72
Calculated Molecular Weight 329 aa, 38 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC040261
Gene Symbol CD86
Gene ID (NCBI) 942
ENSEMBL Gene IDENSG00000114013
RRIDAB_2880677
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42081
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.

实验方案

Product Specific Protocols
WB protocol for CD86 (C-terminal) antibody 26903-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Commun

A photo-triggered self-accelerated nanoplatform for multifunctional image-guided combination cancer immunotherapy

Authors - Xiaoying Kang
ratWB,IF

Int J Biol Macromol

A multifunctional polydopamine/genipin/alendronate nanoparticle licences fibrin hydrogels osteoinductive and immunomodulatory potencies for repairing bone defects

Authors - Wentao Shi
humanWB

Cancer Immunol Immunother

Novel roles of VAT1 expression in the immunosuppressive action of diffuse gliomas.

Authors - Pei Yang
mouseIF

Front Bioeng Biotechnol

Heparin/Collagen-REDV Modification of Expanded Polytetrafluoroethylene Improves Regional Anti-thrombosis and Reduces Foreign Body Reactions in Local Tissues

Authors - Yaping Shan
mouseWB

Biochem Biophys Res Commun

Esomeprazole inhibits endoplasmic reticulum stress and ameliorates myocardial ischemia-reperfusion injury

Authors - Guoxiang Zhou
human,mouseWB,IF

Cell Death Differ

TRAF3/STAT6 axis regulates macrophage polarization and tumor progression

Authors - Jian-Hong Shi