CD22 Monoclonal antibody

CD22 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

7A2F1

Cat No : 66103-1-Ig

Print datasheet

Synonyms

B cell receptor CD22, BL CAM, CD22, CD22 molecule, SIGLEC 2, SIGLEC2, T cell surface antigen Leu 14



经过测试的应用

Positive WB detected inRamos cells, Raji cells, Daudi cells
Positive IHC detected inhuman tonsillitis tissue, human appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue, Raji cells
Planning an IHC experiment? We recommend our IHCeasy CD22 Ready-To-Use IHC Kit. CD22 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2500-1:10000
Immunohistochemistry (IHC)IHC : 1:10000-1:40000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

66103-1-Ig targets CD22 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen CD22 fusion protein Ag17986 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name CD22 molecule
Synonyms B cell receptor CD22, BL CAM, CD22, CD22 molecule, SIGLEC 2, SIGLEC2, T cell surface antigen Leu 14
Calculated Molecular Weight 847 aa, 95 kDa
Observed Molecular Weight 135 kDa
GenBank Accession NumberBC109306
Gene Symbol CD22
Gene ID (NCBI) 933
RRIDAB_2881502
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20273
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.

实验方案

Product Specific Protocols
WB protocol for CD22 antibody 66103-1-IgDownload protocol
IHC protocol for CD22 antibody 66103-1-IgDownload protocol
IF protocol for CD22 antibody 66103-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

J Biomed Res

Causal genetic regulation of DNA replication on immune microenvironment in colorectal tumorigenesis: evidenced by an integrated approach of trans-omics and GWAS

Authors - Sumeng Wang