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Cyclin D1 Monoclonal antibody
Cyclin D1 Monoclonal Antibody for FC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat and More (4)
Applications
FC, WB, ELISA and More (3)
Conjugate
Unconjugated
637
CloneNo.
2G3G5
验证数据展示
经过测试的应用
| Positive WB detected in | U2OS cells, A549 cells, HeLa cells, MCF-7 cells, NIH/3T3 cells, RAW 264.7 cells, SH-SY5Y cells, A431 cells, SW 1990 cells, HepG2 cells, HSC-T6 cells, PC-12 cells, HCT 116 cells, SK-N-SH cells |
| Positive FC detected in | SH-SY5Y cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60186-1-Ig targets Cyclin D1 in WB,FC,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | FC, WB, ELISA |
| Cited Applications | WB,IP,IHC,IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, goat, zebrafish, hamster, bovine |
| Immunogen | Cyclin D1 fusion protein Ag0689 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | cyclin D1 |
| Synonyms | B cell lymphoma 1 protein, CCND1, cyclin D, cyclin D1, D11S287E, G1/S specific cyclin D1, PRAD1, PRAD1 oncogene, U21B31 |
| Calculated Molecular Weight | 295 aa, 34 kDa |
| Observed Molecular Weight | 34 kDa |
| GenBank Accession Number | BC000076 |
| Gene Symbol | CCND1 |
| Gene ID (NCBI) | 595 |
| RRID | AB_10793718 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P24385 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The CCND1 gene, located on 11q13 has been reported to be overexpressed in mantle cell lymphoma (MCL) due to the chromosomal translocation. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Over-expression of CCND1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cyclin D1 antibody 60186-1-Ig | Download protocol |
| FC protocol for Cyclin D1 antibody 60186-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol TRIM15 and CYLD regulate ERK activation via lysine-63-linked polyubiquitination. | ||
Acta Pharm Sin B The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway. | ||
J Clin Invest Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation. | ||
EBioMedicine A miR-567-PIK3AP1-PI3K/AKT-c-Myc feedback loop regulates tumour growth and chemoresistance in gastric cancer. |
