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- KD/KO Validated
c-Cbl Polyclonal antibody
c-Cbl Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IHC, IP, WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, Raji cells, Jurkat cells, Ramos cells, Romas cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human lymphoma tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25818-1-AP targets c-Cbl in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | IHC, IP, WB, ELISA |
| Cited Applications | CoIP, IF, IP, WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | c-Cbl fusion protein Ag23033 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | Cas-Br-M (murine) ecotropic retroviral transforming sequence |
| Synonyms | C CBL, CBL, CBL2, c-Cbl, Proto oncogene c Cbl, RING finger protein 55, RNF55 |
| Calculated Molecular Weight | 906 aa, 100 kDa |
| Observed Molecular Weight | 100-120 kDa |
| GenBank Accession Number | BC132733 |
| Gene Symbol | CBL |
| Gene ID (NCBI) | 867 |
| RRID | AB_2880252 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P22681 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
c-Cbl (Casitas B-lineage lymphoma) is a critical regulator that controls immature or follicular helper T cell development, B cell development, immune tolerance, angiogenesis, or tumorigenesis (PMID: 33962939). c-Cbl also plays a pivotal role in regulating dendritic cell (DC) function via negatively modulating IL-12 production after Toll-like receptor (TLR) stimulation (PMID: 21799517).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for c-Cbl antibody 25818-1-AP | Download protocol |
| IHC protocol for c-Cbl antibody 25818-1-AP | Download protocol |
| IP protocol for c-Cbl antibody 25818-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep Pro-prion, as a membrane adaptor protein for E3 ligase c-Cbl, facilitates the ubiquitination of IGF-1R, promoting melanoma metastasis
| ||
Cell Death Dis ZC3H15 promotes glioblastoma progression through regulating EGFR stability. | ||
Cell Commun Signal Gasdermin E regulates the stability and activation of EGFR in human non-small cell lung cancer cells | ||
J Control Release Imatinib co-loaded targeted realgar nanocrystal for synergistic therapy of chronic myeloid leukemia. |
