• Featured Product
  • KD/KO Validated

BST2 Polyclonal antibody

BST2 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, IF, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 13560-1-AP

Print datasheet

Synonyms

Bone marrow stromal antigen 2, BST 2, BST2, CD317, HM1.24 antigen, Tetherin



经过测试的应用

Positive WB detected inHeLa cells, L02 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13560-1-AP targets BST2 in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IF, IHC, WB, ELISA
Cited Applications CoIP, FC, IF, IHC, WB
Tested Reactivity human
Cited Reactivityhuman
Immunogen BST2 fusion protein Ag4430 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name bone marrow stromal cell antigen 2
Synonyms Bone marrow stromal antigen 2, BST 2, BST2, CD317, HM1.24 antigen, Tetherin
Calculated Molecular Weight 180 aa, 20 kDa
Observed Molecular Weight 30-36 kDa, 50-70 kDa
GenBank Accession NumberBC033873
Gene Symbol BST2
Gene ID (NCBI) 684
RRIDAB_2067220
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ10589
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BST2, also named as CD317 and Tetherin, belongs to the tetherin family. It may be involved in the sorting of secreted proteins and it is involved in pre-B-cell growth. BST2 is an antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. BST2 may play a role in B-cell activation in rheumatoid arthritis. It is recently identified interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. BST2 is a plasma membrane proteins, tetherin inhibits virion particle release from infected cells. BST2 is effective against retroviruses and flavoviruses whilst IFITMs disrupt influenza and flavivirus infection. Observed MW of BST2 is 30-36 kDa (PMID: 19196977; 21237475).

实验方案

Product Specific Protocols
WB protocol for BST2 antibody 13560-1-APDownload protocol
IHC protocol for BST2 antibody 13560-1-APDownload protocol
IF protocol for BST2 antibody 13560-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

PMID: 28965816

Authors - Shouheng Jin
  • KD Validated
humanWB

Proc Natl Acad Sci U S A

PMID: 24753610

Authors - Xuesen Zhao
humanWB, IF

Curr Biol

The viral restriction factor tetherin/BST2 tethers cytokinetic midbody remnants to the cell surface.

Authors - Adrien Presle
  • KO Validated
humanWB

PLoS Pathog

PMID: 28910400

Authors - Yuxuan Fu
  • KO Validated
humanWB, IF

J Virol

Interferon-inducible Protein Tetherin Inhibits Hepatitis B Virus Virion Secretion.

Authors - Ran Yan
  • KD Validated
humanWB

J Virol

Interferon-induced cell membrane proteins, IFITM3 and tetherin, inhibit vesicular stomatitis virus infection via distinct mechanisms.

Authors - Weidner Jessica M JM