Bestrophin-1 Monoclonal antibody

Bestrophin-1 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF, WB, ELISA

Conjugate

Unconjugated

CloneNo.

4D5F1

Cat No : 60326-1-Ig

Print datasheet

Synonyms

ARB, BEST, BEST1, bestrophin 1, Bestrophin-1, BMD, TU15B, VMD2



经过测试的应用

Positive WB detected inY79 cells, Transfected HEK-293 cells
Positive IF detected inY79 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

60326-1-Ig targets Bestrophin-1 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF, WB, ELISA
Cited Applications IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen Bestrophin-1 fusion protein Ag15129 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name bestrophin 1
Synonyms ARB, BEST, BEST1, bestrophin 1, Bestrophin-1, BMD, TU15B, VMD2
Calculated Molecular Weight 585 aa, 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC015220
Gene Symbol BEST1
Gene ID (NCBI) 7439
RRIDAB_2881436
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO76090
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Bestrophin-1 (BEST1) is a 68 kDa transmembrane protein which belongs to the bestrophin family of anion channels. It is predominantly expressed in the basolateral membrane of the retinal pigment epithelium. Studies show that bestrophin 1 functions as a Ca(2+)-dependent Cl(-) channel and modulator of voltage-dependent L-type Ca(2+) channels. It may also contribute to the basolateral cell conductance in airway epithelial cells. This protein is encoded by the VMD2 gene. Mutations in VMD2 can lead to different types of retinal or macular degenerations, including Best vitelliform macular dystrophy (BMD), autosomal recessive bestrophinopathy (ARB), autosomal dominant vitreoretinochoroidopathy (ADVIRC) and adult-onset vitelliform macular dystrophy (AVMD).

实验方案

Product Specific Protocols
WB protocol for Bestrophin-1 antibody 60326-1-IgDownload protocol
IF protocol for Bestrophin-1 antibody 60326-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cell Death Dis

Direct conversion of human umbilical cord mesenchymal stem cells into retinal pigment epithelial cells for treatment of retinal degeneration

Authors - Xiaoman Zhu