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  • Featured Product
  • KD/KO Validated

BDH2 Polyclonal antibody

BDH2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 27207-1-AP

Print datasheet

Synonyms

BDH2, DHRS6, EFA6R, Oxidoreductase UCPA, PRO20933, SDR15C1, UCPA OR, UNQ6308

验证数据展示

  • IHC staining of human breast cancer using 27207-1-AP

    IHC staining of human breast cancer using 27207-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27207-1-AP (BDH2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 27207-1-AP

    IHC staining of human breast cancer using 27207-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27207-1-AP (BDH2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 27207-1-AP

    WB analysis using 27207-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27207-1-AP (BDH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 27207-1-AP

    WB analysis of HeLa using 27207-1-AP

    WB result of BDH2 antibody (27207-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BDH2 transfected HeLa cells.

  • WB analysis using 27207-1-AP

    WB analysis using 27207-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27207-1-AP (BDH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inBxPC-3 cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27207-1-AP targets BDH2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen BDH2 fusion protein Ag24934 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name 3-hydroxybutyrate dehydrogenase, type 2
Synonyms BDH2, DHRS6, EFA6R, Oxidoreductase UCPA, PRO20933, SDR15C1, UCPA OR, UNQ6308
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC095414
Gene Symbol BDH2
Gene ID (NCBI) 56898
RRIDAB_2880800
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BUT1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BDH2 antibody 27207-1-APDownload protocol
IHC protocol for BDH2 antibody 27207-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Clin Invest

Iron-dependent epigenetic modulation promotes pathogenic T cell differentiation in lupus.

Authors - Xiaofei Gao
View Article
human,mouseWB,IHC

J Hematol Oncol

Increased B3GALNT2 in hepatocellular carcinoma promotes macrophage recruitment via reducing acetoacetate secretion and elevating MIF activity.

Authors - Tianxiao Yang
  • KD Validated
View Article
humanIHC

J Exp Clin Cancer Res

BDH2 triggers ROS-induced cell death and autophagy by promoting Nrf2 ubiquitination in gastric cancer.

Authors - Jia-Zhou Liu
View Article