BAD Polyclonal antibody

BAD Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

FC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 10435-1-AP

Print datasheet

Synonyms

BAD, BBC2, BBC6



经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, HepG2 cells, Neuro-2a cells
Positive IHC detected inhuman prostate hyperplasia tissue, human prostate cancer tissue, mouse liver tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:100-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10435-1-AP targets BAD in WB, IHC, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications FC, IHC, WB, ELISA
Cited Applications IHC, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, sheep
Immunogen BAD fusion protein Ag0688 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name BCL2-associated agonist of cell death
Synonyms BAD, BBC2, BBC6
Calculated Molecular Weight 18 kDa
Observed Molecular Weight 18-25 kDa
GenBank Accession NumberBC001901
Gene Symbol BAD
Gene ID (NCBI) 572
RRIDAB_2061994
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92934
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BAD, also named as BBC6 and BCL2L8, promotes cell death. It is successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. BAD can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. It appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

实验方案

Product Specific Protocols
WB protocol for BAD antibody 10435-1-APDownload protocol
IHC protocol for BAD antibody 10435-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

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humanWB

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Oncotarget

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