- Featured Product
- KD/KO Validated
ATM Polyclonal antibody
ATM Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
IF, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human breast cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
27156-1-AP targets ATM in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human samples.
Tested Applications | IF, IHC, IP, WB, ELISA |
Cited Applications | ChIP, IF, IHC, WB |
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Immunogen | ATM fusion protein Ag25909 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ataxia telangiectasia mutated |
Synonyms | A T mutated, AT1, ATA, ataxia telangiectasia mutated, ATC, ATD, ATDC, ATE, ATM, Serine protein kinase ATM, TEL1, TELO1 |
Observed Molecular Weight | 310-350 kDa |
GenBank Accession Number | BC137169 |
Gene Symbol | ATM |
Gene ID (NCBI) | 472 |
RRID | AB_2880780 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q13315 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for ATM antibody 27156-1-AP | Download protocol |
IHC protocol for ATM antibody 27156-1-AP | Download protocol |
IF protocol for ATM antibody 27156-1-AP | Download protocol |
IP protocol for ATM antibody 27156-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Sci Transl Med Guanosine diphosphate-mannose suppresses homologous recombination repair and potentiates antitumor immunity in triple-negative breast cancer | ||
Oncogene TRIM24 is critical for the cellular response to DNA double-strand breaks through regulating the recruitment of MRN complex | ||
Clin Transl Med Single-cell profiling-guided combination therapy of c-Fos and histone deacetylase inhibitors in diffuse large B-cell lymphoma. | ||
EBioMedicine MAP4K1 functions as a tumor promotor and drug mediator for AML via modulation of DNA damage/repair system and MAPK pathway. | ||
Oncotarget 2-Acetylamino-3-[4-(2-acetylamino-2-carboxyethylsulfanylcarbonylamino) phenyl carbamoylsulfanyl] propionic acid, a glutathione reductase inhibitor, induces G2/M cell cycle arrest through generation of thiol oxidative stress in human esophageal cancer cells. |