AARE Polyclonal antibody
AARE Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | COLO 320 cells, HeLa cells, human plasma tissue |
Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
14758-1-AP targets AARE in WB, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | IHC, WB, ELISA |
Cited Applications | WB |
Tested Reactivity | human |
Immunogen | AARE fusion protein Ag6511 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | N-acylaminoacyl-peptide hydrolase |
Synonyms | AARE, ACPH, Acyl peptide hydrolase, Acylaminoacyl peptidase, APEH, APH, D3F15S2, D3S48E, DNF15S2, OPH, Oxidized protein hydrolase |
Calculated Molecular Weight | 81 kDa |
Observed Molecular Weight | 66-80 kDa |
GenBank Accession Number | BC000362 |
Gene Symbol | APEH |
Gene ID (NCBI) | 327 |
RRID | AB_2057811 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P13798 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
APH, (APEH, acylpeptide hydrolase), which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. A range of molecular masses have been reported for APH by previous investigators, including 80 kD, 75 kD and 69 kD. This antibody can recognize at least 2 isoforms of APH.
实验方案
Product Specific Protocols | |
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WB protocol for AARE antibody 14758-1-AP | Download protocol |
IHC protocol for AARE antibody 14758-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
mSphere Internalization of Erythrocyte Acylpeptide Hydrolase Is Required for Asexual Replication of Plasmodium falciparum. |