Albumin Monoclonal antibody

Albumin Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig and More (2)

Applications

IF, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

4A1C11

Cat No : 66051-1-Ig

Print datasheet

Synonyms

Alb, albumin, HSA, Serum albumin



经过测试的应用

Positive WB detected inhuman plasma tissue, human liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ALB Ready-To-Use IHC Kit. ALB primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 750 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66051-1-Ig targets Albumin in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, rat, pig samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications IF, IHC, IP, WB
Tested Reactivity human, rat, pig
Cited Reactivityhuman, mouse, rat, rabbit, pig
Immunogen Albumin fusion protein Ag9885 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name albumin
Synonyms Alb, albumin, HSA, Serum albumin
Calculated Molecular Weight 609 aa, 69 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC034023
Gene Symbol ALB
Gene ID (NCBI) 213
RRIDAB_11042320
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP02768
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. (21388516, 23832071) In additon, glycated serum albumin is also a potential diabetes biomarker.

实验方案

Product Specific Protocols
WB protocol for Albumin antibody 66051-1-IgDownload protocol
IHC protocol for Albumin antibody 66051-1-IgDownload protocol
IF protocol for Albumin antibody 66051-1-IgDownload protocol
IP protocol for Albumin antibody 66051-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Metab

Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.

Authors - Xiao Wang
mouseWB

Cell Metab

TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.

Authors - Dong Huang
humanIF

J Hepatol

Targeting NFATc4 attenuates non-alcoholic steatohepatitis in mice.

Authors - Meng Du
pigIF

Sci Bull (Beijing)

Single-cell dynamics of liver development in postnatal pigs

Authors - Lin Rao
humanWB

J Extracell Vesicles

Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.

Authors - Xiaohan Gao
humanWB

Small

Persistency of Enlarged Autolysosomes Underscores Nanoparticle-Induced Autophagy in Hepatocytes.

Authors - Ji-Qian Zhang