ACPP Polyclonal antibody

ACPP Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

IF, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 15840-1-AP

Print datasheet

Synonyms

acid phosphatase, prostate, ACP 3, ACP3, ACPP, PAP, Prostatic acid phosphatase



经过测试的应用

Positive WB detected inPC-3 cells, COLO 320 cells, MCF-7 cells, mouse lung tissue, mouse testis tissue
Positive IHC detected inhuman prostate cancer tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy PAP/ACPP Ready-To-Use IHC Kit. PAP/ACPP primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15840-1-AP targets ACPP in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF, IHC, WB, ELISA
Cited ApplicationsIF, IHC, IP, WB
Tested Reactivity human, mouse
Cited Reactivitymouse, rat, chicken
Immunogen ACPP fusion protein Ag8592 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name acid phosphatase, prostate
Synonyms acid phosphatase, prostate, ACP 3, ACP3, ACPP, PAP, Prostatic acid phosphatase
Calculated Molecular Weight 418 aa, 48 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC007460
Gene Symbol ACPP
Gene ID (NCBI) 55
RRIDAB_2222099
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15309
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACPP is also named as PAP(prostatic acid phosphatase),5'-nucleotidase, thiamine monophosphatas and Belongs to the histidine acid phosphatase family.This enzyme is a homodimeric glycoprotein with a subunit molecular mass of 50 kDa and is an androgen-stimulated secretory product of the human prostate gland(PMID:8132635).It is also a non-specific phosphatase and has been implicated in the progression of prostate cancer, which inactivates LPA in human seminal plasma(PMID:15280042). This protein has three glycosylation sites(PMID:10639192;12525165).

实验方案

Product Specific Protocols
WB protocol for ACPP antibody 15840-1-APDownload protocol
IHC protocol for ACPP antibody 15840-1-APDownload protocol
IF protocol for ACPP antibody 15840-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
chickenIHC

Biol Reprod

Avian Prostatic Acid Phosphatase: Estrogen Regulation in the Oviduct and Epithelial Cell-Derived Ovarian Carcinomas.

Authors - Hyocheol Bae
mouseIHC

Am J Pathol

PIK3CG Is a Potential Therapeutic Target in Androgen Receptor-Indifferent Metastatic Prostate Cancer.

Authors - Wen-Cheng Chung
mouseWB,IF,IP

Eur J Pain

Electroacupuncture relieves neuropathic pain by inhibiting degradation of the ecto-nucleotidase PAP in the dorsal root ganglions of CCI mice.

Authors - Xiaoxin Zhou
RatIHC, IF

Biochem Biophys Res Commun

Dietary cholesterol affects expression of prostatic acid phosphatase in reproductive organs of male rats.

Authors - Whasun Lim